Journal: bioRxiv

Article Title: hsa-miR-9-5p highly expressed in syncytiotrophoblast-derived extracellular vesicles from early-onset preeclampsia impairs cerebral microvascular endothelial cell pro-angiogenic capacity

doi: 10.1101/2024.10.21.619546

Figure Lengend Snippet: A) Representative image of the Proteome Profiler™ Human Angiogenesis Antibody Array in hCMEC/D3 treated (48 h) with mirVana™ miRNA Mimic, Negative Control (Scramble), or hsa-miR-9-5p (10 nM). Left image densitometry of the representative blots. Right images represent ImageJ conversion in arbitrary color scale (white to blue, representing low and high expression respectively). B) Log2 fold of change of differentially expressed proteins. Scatter line represent 0.5 Log fold of change. C) Protein-protein interaction (PPI) network construction and cluster analysis as described in methods section. D) VCAM-1 protein levels in cell culture supernatants of hCMEC/D3 after stimulation with tumor necrosis factor-α (TNF-α, of 10 ng/mL for 16 hours) in presence of hsa-miR-9-5p (10 nM). The control groups received an equivalent volume of fresh supplemented EGM-2 growth medium with and without scramble construct. VCAM-1: vascular cell adhesion molecule 1, NS, non-significant differences. ** p <0.01. *** p <0.001; **** p <0.0001.

Article Snippet: The expression patterns of proteins associated with angiogenesis were assessed utilizing the Proteome Profiler™ Human Angiogenesis Antibody Array Kit (R&D Systems, Minneapolis, MN, USA).

Techniques: Ab Array, Negative Control, Expressing, Cell Culture, Control, Construct